In vitro propagation of threatened therapeutic tree Buchanania lanzan Spreng (Anacardiaceae)

Embryo rescue refers to an in vitro technology have been used for propagating plants under from poorly developed embryos by avoiding embryo abortion. The aim of the present study was regeneration a medicinally wild tree species Buchanania lanzan through method. Isolated immature dissected out de-coded seeds collected 15 years old trees were cultured Woody Plant Medium (WPM) test germination. 100% germination recorded when inoculated on medium supplemented with 1.5 mg/L-1 6-benzylaminopurine (BAP) and 50 adenine sulphate (AS). Further, week-old seedling derived explants viz axis cotyledonary node selected clonal propagation. On augmented showed maximum mean shoots numbers (4.2±1.80) 4.0±0.98 cm shoot height achieved node. Compare Kinetin (Kn) BAP be better shooting response multiple induction. Healthy regenerated separated cluster transferred rooting containing half strength WP fortified Indole-3-butyric acid (IBA). IBA at 0.5 90% roots (4.9±0.98) per shootlet obtained. rooted plantlets polythene bags autoclaved humus, red soil farmyard manure (1:1:1 ratio) acclimation 25 ± 2 ᵒC. Later, these gradually field condition. About 70% survival rate after three months period. protocol restore viable rapid multiplication can help ex situ conservation this threatened B. lanzan.
 Keywords: plant medium, In seedlings, 6-benzylaminopurine, Adenine sulphate,